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. 2017 Nov 20;128(1):207–218. doi: 10.1172/JCI94955

Figure 5. Pre- and perinatal osteoblasts tolerate SS18-SSX2 expression, but render dense and disorganized skeletal phenotypes.

Figure 5

(A) H&E histology photomicrographs of tibiae from control and OcCre hSS2 embryos harvested 18.5 days postcoitum show dense, woven bone replacing the medullary canal in the latter. (B) Higher-power photomicrographs from the same groups show the replacement of hematopoietic progenitor cells with spindle-shaped osteoblasts in the latter. Photomicrographs of anti-EGFP IHC in a Myf5Cre hSS2 tumor (C, positive control) and bone sections from (D) an OcCre hSS2 embryo and (E) the Col1a1Cre hSS2 mouse that survived to 6 months, showing fusion expression in osteoblasts in the latter 2. (F) Micro-CT axial, coronal, and sagittal images of the tibia from a control mouse and the only surviving Col1a1Cre hSS2 mouse at 6 months of age, showing short, dense, and poorly remodeled bones in the latter. (G) H&E histology photomicrographs of cortical bone from the same mice as in F reveal contrasting lamellar control bone in the former and disorganized ossification patterns in the latter. Scale bars (A, B, G) and panel widths: 100 μm (C–F).