Skip to main content
. 2017 Dec 27;11:103–112. doi: 10.2147/OTT.S147184

Figure 2.

Figure 2

ROCK1 was a direct target of miR-199a in A498 cells.

Notes: (A) Sequence alignment of miR-199a and ROCK1 3′-UTR, which contains one predicted miR-199a-binding site. The seed-recognizing sites in the ROCK1 3′-UTR is indicated in red, while the ROCK1 Mut 3′-UTR is indicated in blue. (B) Luciferase assay in A498 cells co-transfected with miR-199a and luciferase reporter containing ROCK1 WT or a Mut 3′-UTR. (C) miR-199a transfection affects ROCK1 mRNA levels. *P<0.05. (D) miR-199a transfection affects ROCK1 protein levels.

Abbreviations: ctrl, control; Mut, mutant; ROCK1, Rho-associated coiled coil-containing protein kinase 1; WT, wildtype.