Figure 5.

Snail regulated miR-199a expression.

Notes: (A) The putative binding sites of transcription factor Snail in the DNA region of miR-199a. (B) The pGL3-miR-199a pro vector was capable of directing luciferase expression. (C) Luciferase activity was efficiently repressed when pGL3-miR-199a pro vector was co-transfected with pcDNA.3-Snail. (D) The qRT-PCR and Northern blot analysis showed that Snail decreased miR-199a expression. (E) Inverse correlation between miR-199a expression and Snail mRNA level in primary ccRCC tissues. *P<0.05.

Abbreviations: Luc, luciferase; qRT-PCR, quantitative real-time PCR; ccRCC, clear cell renal cell carcinoma.