Figure 3.

Lymphocytic choriomeningitis virus (LCMV)-induced hemophagocytic lymphohistiocytosis (HLH) model comparing nontransplanted wild-type and Prf1^−/− mice versus those transplanted with wild-type and Prf1^−/− LSK cells. (a) Cytotoxic function analysis of splenocytes 8 days after LCMV challenge, using a ⁵¹Cr release assay with GP-33-loaded EL4 cell targets. Symbols represent means and error bars represent the SEM from four mice per group (three mice in the PGK4T group). (b) IFN-γ levels were measured in peripheral blood 8 days after LCMV infection. Statistics were done by one-way Dunnett's analysis of variance, comparing all groups with the wild-type mice. (c) Mice challenged with LCMV were clinically scored two or three times per week. Clinical scoring is represented for groups until they had ≥10% survival rate (n = 6 experiments; wild-type, n = 16; TxWT, n = 22; Prf1^−/−, n = 16; TxPrf1^−/−, n = 12 mice). (d) Survival of LCMV-challenged mice (n = 6 experiments; wild-type, n = 16; TxWT, n = 22; Prf1^−/−, n = 20; TxPrf1^−/−, n = 12 mice). Statistics were done by Mantel–Cox test. Error bars represent the SEM. ^†Death of all mice in that group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.