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. Author manuscript; available in PMC: 2018 Jan 2.
Published in final edited form as: Biochemistry. 2016 Jul 6;55(28):3995–4002. doi: 10.1021/acs.biochem.6b00467

Figure 5.

Figure 5

Putative regulator binding pocket on CVB3 3Dpol based on loss of allosteric regulation by ATP in H228A/E, I230F, and F232Y mutants. A) Structure of the stalled CVB3 3Dpol elongation complex (PDB:4K4Y) with a bound sulfate from the apo-3Dpol structure (PDB:3DDK). Motif A residues lining the putative allosteric pocket are colored orange, motif D is colored dark green, and Phe364 is marked in lighter green. B) Detailed view of the mutated residues and their location relative to the active site with a bound dideoxy-CTP. Mutated motif A residues are shown as orange spheres and Phe364 in motif D is shown in light green sticks.