Table 1.
Globala
|
Graphical b
|
|||||||
---|---|---|---|---|---|---|---|---|
Wild Type | Wild Type | F364W | I230F | F232Y | H228A | H228E | M310W | |
|
|
|||||||
Kic(GTP) | 590 ± 80 | 440 ± 60 | 490 ± 60 | 360 ± 80 | 460 ± 80 | 380 ± 90 | 350 ± 80 | 350 ± 75 |
Kic(UTP) | 3100 ± 800 | 2900 ± 300 | n.d.c | n.d. | n.d. | n.d. | n.d. | n.d. |
Kic(ATP) | 1100 ± 200 | 1500 ± 200 | 1300 ± 100 | 800 ± 200 | 900 ± 200 | 1400 ± 200 | 1900 ± 300 | 1000 ± 110 |
Kiu(ATP) | 1800 ± 100 | 1900 ± 300 | 2200 ± 400 | n.a.d | 3700 ± 900 | n.a. | 3900 ± 1000 | 2900 ± 400 |
All units are μM.
Global fitting with KinTek Explorer software with standard errors.
Graphical analysis via Dixon and Cornish-Bowden plots with standard error of the fitted parameters.
n.d. = UTP inhibition constants were not determined for the mutant polymerases.
n.a. = uncompetitive inhibition was not observed.