The electrostatic free energy ΔG as a function of [Mg2+] for (from top to bottom) the rRNA with 20mM K+ (blue line), rRNA with 60 mM K+ (cyan line), adenine riboswitch with 50 mM K+ (green line), 76-nt yeast tRNAPhe with 32mM Na+ (red line), the 80-nt RNA duplex with 10 mM Na+ (black line), rRNA-protein complex with 60 mM K+ (magenta line), respectively.