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. 2017 Oct 16;7(2):e1382790. doi: 10.1080/2162402X.2017.1382790

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 5. — SIRT1 interacts with and deacetylates hMOF. (A) Immunoblot analysis of SIRT1 and hMOF in BV2 microglia cultured for 4 h as monoculture or with C6 glioma cells as segregated coculture assessed after immunoprecipitation with antibody to SIRT1 or immunoglobulin G (IgG; control). IgG bands are indicated with an asterisk, whereas proteins of interest are indicated with an arrow head. (B) In situ proximity-ligation assay (PLA) with quantifications (C) of SIRT1-hMOF interactions in BV2 microglia grown for 4 h as monocultures or with C6 cells as segregated cocultures; results are presented relative to those of BV2 monocultures, set as 1. (D) Immunoblot analysis of hMOF in BV2 microglia cultured for 4 h as monoculture or with C6 glioma cells as segregated coculture assessed after immunoprecipitation with antibody to acetyl lysine (acK) or immunoglobulin G (IgG; control). (E) In situ proximity-ligation assay (PLA) with quantification (F) of acK-hMOF interactions in BV2 microglia grown for 4 h as monocultures or with C6 cells as segregated cocultures; results are presented relative to those of BV2 monocultures, set as 1. **P < 0.01and ***P < 0.001 (two-tailed Student's t-test). Data are from three independent experiments (mean and s.d.).