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. 2017 Oct 16;7(2):e1382790. doi: 10.1080/2162402X.2017.1382790

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© 2018 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 6. — The H4K16 HAT hMOF is recruited to microglial upregulated genes. BV2 microglia are cultured for 4 h as monocultures or with C6 glioma cells as segregated cocultures and immunoprecipitated with hMOF (A, C, E, G and I) or H4K16ac (B, D, F, H and J) antibodies. hMOF and H4K16ac ChIP signals in 1 kb region downstream of the transcription start sites for the genes Il1β, Il6, Mmp14, Ccl22 and Chil3 were analyzed using qPCR and normalized to the levels of input DNA. BV2 microglia grown as monocultures serves as a control, set as 1. *P < 0.05 and **P < 0.01 (two-tailed Student's t-test). Data are from three (Ccl22 and Chil3) or five (Il1β, Il6 and Mmp14) independent experiments (mean and s.d.).