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. 2017 Nov 27;7(2):e1364827. doi: 10.1080/2162402X.2017.1364827

Figure 1.

Figure 1.

Platelet-induced shedding of NKG2DL impairs NK antitumor reactivity. The indicated tumor cells were cultured for 48 h alone or in the presence of platelets (ratio tumor cells/platelets of 1:150) to enable coating. After washing to remove surplus platelets and soluble factors, (A) surface expression of MICA, MICB, ULBP1–3 and EGFR/CD133 as control were analyzed by flow cytometry. Open peaks, tumor cells alone; shaded peaks, platelet-coated tumor cells; dotted lines, isotype controls. (B) Levels of sNKG2DL in supernatants were analyzed by ELISA. Statistically significantly different results are indicated by *. (C) Tumor cells were incubated with pNKC in the presence or absence of anti-NKG2D F(ab´)2 fragments or isotype control (both 5 µg/ml). Target cell lysis was determined by 4 h chromium release assays. Results obtained at the indicated E:T ratios (left) and the calculated reduction of lysis (δ) by NKG2D blockade (at the lowest E:T ratio, right) are displayed. Representative data of one experiment from a total of at least 4 with similar results are shown.