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. 2017 Nov 1;7(2):e1390641. doi: 10.1080/2162402X.2017.1390641

Figure 4.

Figure 4.

PD-L2-specific T cells are effector T cells. (A) Intracellular cytokine staining showing CD4+ and CD8+ T cells that release TNF-α in response to either an irrelevant control peptide HIV peptide (HIV-1 pol476-484) or PD-L205 (PD-L216-25) in cultures of PD-L2T cells-A (left,) and PD-L2T cells-B (right). (B) Intracellular TNF-α and IFN- γ cytokine staining of PD-L2T-cells culture-A (left) and PD-L2T-cells culture-B (right) in response to 5 hours stimulation with autologous DCs. (C) IFN-γ and TNF-α secretion by PD-L2T-cell culture-A (top) and PD-L2T-cell culture-B (bottom) towards PD-L205 (PD-L216-25) peptide (black bars) and autologous DCs when cultured at ratio 1:5 (grey bars) as measured by ELISPOT assay. (D) T2 cells pulsed either with PD-L205 (PD-L216-25) or a control HIV peptide (HIV-1 pol476-484) as recognized by by PD-L2T-cell culture-A (left) and PD-L2T-cell culture-B (right) in a standard 51Cr-release assay.