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. 2017 Dec 18;7(2):e1393599. doi: 10.1080/2162402X.2017.1393599

Figure 4.

Figure 4.

Immunosuppressive activity of monocyte subsets against CD8 T cells. (A) CD8 T cell proliferation shown as division index, with or without 3 days stimulation with CD3/CD28 beads and co-culture with tumor-derived Ly6Chi or Ly6Clo monocytes at the indicated monocyte to CD8 T cell ratios. Histograms show representative proliferation (CTV dye dilution) of CD8 T cells cultured in presence of absence of monocytes. (B/C) Percent suppression of CD8 T cell proliferation by Ly6Chi and Ly6Clo monocytes derived from healthy (naïve) or Eµ-myc tumor-bearing mice at 1 monocyte : 1 T cell ratio. (D) Percent suppression of PD-1 positive and PD-1 negative CD8 T cell proliferation in the presence of tumor-derived Ly6Chi and Ly6Clo monocytes at 1:1 ratio. (* p < 0.05; ** p < 0.01; n = 5 per group).