Table 2. IC50 values of compounds 4–12 against ATP hydrolysis activity of fungal and mammalian P-type ATPases.
n = 3.
| ID | ATP hydrolysis IC50 [μM] | |||
|---|---|---|---|---|
| Saccharomyces cerevisiae Pma1 | Candida albicans Pma1 | Mammalian SERCA | Mammalian Na+,K+-ATPase | |
| 4 | 75.0 ±3.2 | 46.0 ±14.5 | 1.55 ±0.06 | 37.2 ±13.6 |
| 5 | 85.9 ±10.3 | 129.7 ±32.2 | 61.7 ±18.8 | 131.9 ±35.2 |
| 6 | 2.79 ±0.36 | 3.89 ±1.71 | 0.09 ±0.03 | 0.72 ±0.30 |
| 7 | 2.43 ±0.39 | 2.47 ±0.26 | 0.11 ±0.06 | 0.38 ±0.12 |
| 8 | 3.57 ±1.81 | 3.77 ±1.04 | 0.26 ±0.06 | 1.07 ±0.38 |
| 9 | 4.73 ±2.23 | 5.56 ±1.55 | 3.27 ±1.89 | 9.76 ±4.47 |
| 10 | 6.69 ±2.06 | 9.70 ±2.15 | 5.54 ±3.16 | 5.50 ±2.74 |
| 11a | >20 | >20 | >20 | >20 |
| 12a | >20 | >20 | >20 | 11.8 ±6.8 |
aThe compounds were tested in a concentration up to 166 μM, but compound precipitation was observed at concentrations above 20 μM.