Fig 5. HIV transmission between primary myeloid DCs and CD4⁺ T cells is capable of drug-insensitivity.

Primary myeloid DCs (mDCs) were cocultured with autologous CD4⁺ T cells at ratios of 1:4 and 1:8. (A) The mDC-CD4⁺ T cell cocultures and DC-free CD4⁺ T cell cultures were infected with NL4-3 and the frequency of infected p24⁺ T cells was measured using flow cytometry. (B) The mDC-CD4⁺ T cell cocultures and DC-free CD4⁺ T cell cultures were infected with NL4-3 in the presence or absence of 10 μM of TFV and drug insensitivity (T_x) was measured. (C) Fold difference between the T_x values of mDC-CD4⁺ T cell coculture and DC-free culture were measured. Mean ± s.e.m (η = 3 technical replicates). *, p <0.05 (student’s T-test); n.s., p >0.05. Data is representative of one donor from two independent experiments (A-C).