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. 2018 Jan 2;13(1):e0190026. doi: 10.1371/journal.pone.0190026

Fig 8. Pre-treatment of V. cholerae with ZAC-3 IgG results in resistance to secondary complement-mediated lysis.

Fig 8

(A) CV staining of mid-log phase V. cholerae O395 following treatment for 1 h with SyH7 F(ab’)2, 2D6 F(ab’)2, ZAC-3 F(ab’)2 at (9 μg/mL), or 2D6 IgA (36μg/mL). (B) Complement-mediated lysis of V. cholerae O395 following the pre-treatments described in Panel A. Each bar constitutes the combined results from at least three biological replicates. In both panels, statistical significance across treatments was determined by one way ANOVA, followed by a Tukey multiple comparison test. ns, not significant. *; P< 0.05.