Table 1. Candidate genes, primers, and different parameters derived from the qRT-PCR analysis.
| Gene | Gene symbol | Primer sequences (5’-3’) | Amplicon Size (bp) | Tm (oC) | E (%) | Regression coefficient (R2) |
|---|---|---|---|---|---|---|
| 18S ribosomal protein | 18S | For GCGCTACACTGACAGAGCCA | 178 | 82.4±0.22 | 110 | 0.981 |
| Rev GCGGTGTGTACAAAGGGCAG | ||||||
| 28S ribosomal protein | 28S | For TGGCTCTAAGGGTTGGGTGC | 129 | 82.0±0.19 | 93 | 0.996 |
| Rev CCCGAAGAGCAGCCAAAGTC | ||||||
| plasma membrane proton ATPase | Pma | For GGTGCGTTCTGTGTACCCCA | 157 | 81.2±0.25 | 97 | 0.994 |
| Rev TCGCGAACTTGATCCAGTCGA | ||||||
| α-Tubulin | Atu | For ATCTGTAACGAACCCCCAGC | 169 | 83.3±0.35 | 96 | 0.946 |
| Rev CACCGACGTACCAGTGAACA | ||||||
| β-tubulin | Btu | For CAGTTCACGGCCATGTTCA | 152 | 82.5±0.16 | 91 | 0.965 |
| Rev CGACGGTGGCATCCTGGTA | ||||||
| adenine phosphoribosyl transferase | Apt | For AGGAAGTGAAATGGCCGAGG | 161 | 82.0±0.08 | 101 | 0.963 |
| Rev TGATGCGAAGAGCCCATTCA | ||||||
| ribosomal protein L4 | Rpl4 | For AATCGTAGACACCGTCAGCG | 160 | 82.5±0.00 | 110 | 0.938 |
| Rev TGACGAAACGGCCAAGATGA | ||||||
| ribosomal protein L2 | Rpl2 | For AATCGATCCCTCGGGAAAGC | 181 | 84.5±0.11 | 95 | 0.974 |
| Rev CAGGCTTCCTTGTCCTCGTT | ||||||
| tryptophan synthase-β | Tsb | For CTGGTGTTGGACCTGAGCAT | 183 | 82.6±0.15 | 99 | 0.986 |
| Rev CCTTAGGAAGCGTCTTGGCA | ||||||
| UDP-glucose pyrophosphorylase | UGPase | For GACGGCCAAGGGGTTATTCA | 165 | 83.0±0.00 | 110 | 0.979 |
| Rev TTGACCGTGGCTCAAAGAGT | ||||||
| phosphoglucose isomerase | PGI | For TCCATCAGGGCACCAAACTC | 141 | 82.0±0.00 | 109 | 0.981 |
| Rev CGGTCTTACCGAAGGCCAAT | ||||||
| phosphoglucomutase | PGM | For CCCATGCCGACGAATACAGA | 176 | 84.5±0.00 | 96 | 0.985 |
| Rev GGTGTGAGCGTAGGTCAAGT |