Table 2. Overall ranking of the candidate reference genes for the four sample groups after geNorm, NormFinder, and BestKeeper analysis.
| Candidate reference genes | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Rpl4 | Btu | Rpl2 | Tsb | Atu | Apt | 18s | 28s | Pma | F-actin | ||
| Strain | geNorm | 1 | 3 | 5 | 6 | 2 | 7 | 4 | 9 | 10 | 8 |
| Normfinder | 2 | 3 | 6 | 7 | 1 | 5 | 4 | 9 | 10 | 8 | |
| BestKeeper | 7 | 5 | 3 | 2 | 6 | 9 | 4 | 1 | 10 | 8 | |
| Total score | 10 | 11 | 14 | 15 | 9 | 21 | 12 | 19 | 30 | 24 | |
| Overall rank* | 2 | 3 | 5 | 6 | 1 | 8 | 4 | 7 | 10 | 9 | |
| Development | geNorm | 7 | 3 | 4 | 6 | 8 | 9 | 1 | 2 | 5 | 10 |
| Normfinder | 7 | 3 | 3 | 6 | 8 | 9 | 2 | 1 | 5 | 10 | |
| BestKeeper | 3 | 4 | 6 | 9 | 1 | 2 | 5 | 8 | 7 | 10 | |
| Total score | 17 | 10 | 13 | 21 | 17 | 20 | 8 | 11 | 17 | 30 | |
| Overall rank | 5 | 2 | 4 | 9 | 5 | 8 | 1 | 3 | 5 | 10 | |
| Nutrient | geNorm | 1 | 3 | 5 | 4 | 2 | 7 | 6 | 10 | 8 | 9 |
| Normfinder | 1 | 3 | 6 | 5 | 2 | 7 | 4 | 10 | 8 | 9 | |
| BestKeeper | 5 | 7 | 2 | 3 | 4 | 8 | 6 | 1 | 9 | 10 | |
| Total score | 7 | 13 | 13 | 12 | 8 | 22 | 16 | 21 | 25 | 28 | |
| Overall rank | 1 | 4 | 4 | 3 | 2 | 8 | 6 | 7 | 9 | 10 | |
| All | geNorm | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Normfinder | 1 | 2 | 4 | 5 | 6 | 3 | 7 | 8 | 9 | 10 | |
| BestKeeper | 5 | 6 | 3 | 2 | 4 | 8 | 7 | 1 | 9 | 10 | |
| Total score | 7 | 10 | 10 | 11 | 15 | 17 | 21 | 17 | 27 | 30 | |
| Overall rank | 1 | 2 | 2 | 4 | 5 | 7 | 8 | 6 | 9 | 10 | |
*The overall rank is expressed in boldface.