Table 2. Transformation efficiency of ‘Hamlin’ sweet orange from 1 gram of suspension cell derived cultures.
Transformation system |
VvmybA1-negative embryos a | VvmybA1-positive embryos a |
VvmybA1-positive shoots (Plant recovery efficiency (%))b |
---|---|---|---|
Suspension Cells | 74 | 56 | 18 (32) |
Protoplast | 194 | 21 | 11 (52) |
a Data from three independent experiments. Results indicate the total number of embryos produced (VvmybA1 positive and negative)
b Plant recovery efficiency was calculated as the total number of VvmybA1-positive shoots/total number of VvmybA1-positive embryos produced X 100.