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. 2018 Jan 2;13(1):e0190513. doi: 10.1371/journal.pone.0190513

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© 2018 Stockum et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — 293T cells were transfected with plasmids expressing the indicated Flag- or HA-tagged fusion proteins. “–” refers to empty plasmid control. Input and Flag-IP samples are indicated underneath each panel. Antibodies and fusion proteins are indicated to the right of each western blot. (A) Flag-IP with Flag-tagged USP11 recovers HA-tagged RAE1, (B) HA-KCTD6 and (C) HA-SPRYD3. (D) HA-USP11 co-precipitates with Flag-SPRYD3. (E) Flag-tagged RAE1 and KCTD6 co-precipitate HA-tagged USP11. (F) Endogenous RAE1, PAM and SPRYD3 co-precipitate with Flag-USP11, whilst no CIT can be detected in the IP western blot. 1% of the extract was loaded in the input; the IP represents 10% of the input. Molecular weight markers are indicated to the left of the western blots. The data shown are representative results from three biological replicates.