Table 2. Antibodies employed for flow cytometry analysis and sorting.
Antibody Target | Conjugated Fluorophore | Host Species | Isotype | Clone | Manufacturer | Catalogue Number |
---|---|---|---|---|---|---|
CD4 | BB515 | Mouse | IgG1κ | RPA-T4 | BD Biosciences | 564419 |
CD45RA | APC-H7 | Mouse | IgG2b κ | HI100 | BD Biosciences | 560674 |
PD1 | PE | Mouse | IgG1 κ | MIH4 | BD Biosciences | 557946 |
CXCR5 | BV421 | Rat | IgG2b κ | RF8B2 | BD Biosciences | 562747 |
CCR6 | BV711 | Mouse | IgG1 κ | IIA9 | BD Biosciences | 563923 |
CXCR3 | BUV395 | Mouse | IgG1 κ | IC6 | BD Biosciences | 565223 |
CXCR5 | BV711 | Mouse | IgG1 κ | J252D4 | Biolegend | 356934 |
FOXP3 | BV421 | Mouse | IgG1 κ | 206D | Biolegend | 320124 |