Table 3. Sequences of RT-qPCR oligonucleotide primers and probes designed on AOX gene sequences.
AS: amplicon size.
| Species | Accession ID | Primers (5’→ 3’) | Probes (5’→ 3’) | As(bp) |
|---|---|---|---|---|
| Maize | ZM05G37570 |
Fw: GCCCTCTGAGTTCCTTTGCTT Rv: GAGCTGCATACCCTGGAAATG |
TGATGCGCAGGACAT | 92 |
| Barley | CAJW010038523 |
Fw: TTGATTCTGAACTCCTGACGATGT Rv: CACGTCCTGCACGACACAAT |
TGCTGATCTGGTCTGTC | 63 |
| Soybean | NM_001248837.1 |
Fw: ATGAAGCTCACTGCACTGAATTCT Rv: GGCGGTTACCGTTTTGGTT |
CTGCTCAATGGCC | 73 |
| Wheat | TRAES3BF079200040CFD |
Fw: GCTACCACTGAGGAAAGGGACTAC Rv: TGCATCCAAACAAGCTCCAA |
CCGTACGCAATAAA | 63 |
| Flax | MF281398 |
Fw: TCGCATTCTGGTCTGTCAAATC Rv: AAGGGAGTAACCTGAAAGAACAAGTC |
CTCCGCTGGCCAAC | 64 |
| Sunflower | MF281399 |
Fw: TCCAGCACCTGTGGGTTACC Rv: ACCCATACCACCAGAATCCTATACC |
AAACCGTTTGCTTATAGAGG | 69 |
| Peanut | MF281397 |
Fw: TTTTTTGGAAGATCTGGTCCAGTT Rv: TACGAGGCAAAAAATCAGGTACAG |
CTTAACCTAAAGTTTCAGCTGAT | 128 |
| Rice | OSINDICA_02G20320 |
Fw: ATCGCCATTAAGGGATCAATAAATA Rv: CATTGCATGCGTCCAAATAATAC |
CCGGAGACAATAAT | 67 |
| Cotton | MF281396 |
Fw: GACGGCTCCCCATGGAA Rv: TGTTGCACGAAAAAGACCAATT |
TGGACCTGCTTTAGGG | 63 |