Table 8. Results of the ILC test using TaqMan assay and end-point PCR methodology based on AOX genes.
End-point results are reported in Braglia et al. (2017). TCN: target copy number; D: detected; ND: not detected.
| Mix | Species present | %w/w | AOX TaqMan probes | AOX end-point probes (D/traces/ND) | |
|---|---|---|---|---|---|
| Cq value | TCN80 | ||||
| ILC1 | Maize | 50 | 25.48 | 5647.0 | D |
| Soybean | 34.8 | 26.17 | 7867.8 | D | |
| Sunflower | 8 | 27.12 | 30973.5 | D | |
| Flax | 7.5 | 27.56 | 27999.6 | D | |
| ILC2 | Durum+soft wheat | 45 | 25.89 | 18735.3 | D |
| Maize | 12 | 28.15 | 859.4 | D | |
| Barley | 15 | 26.99 | 1749.7 | D | |
| Soybean | 18 | 28.39 | 1620.1 | D | |
| Rice | 10 | 29.44 | 312.4 | D | |
| ILC3 | Barley | 100 | 25.04 | 7325.6 | D |
| ILC4 | Durum+soft wheat | 20 | 28.63 | 3386.1 | D |
| Maize | 55 | 27.28 | 1688.2 | D | |
| Sunflower | 10 | 31.31 | 2472.8 | ND | |
| Cotton | 15 | 27.91 | 1972.4 | D | |
| ILC5 | Durum+soft wheat | 100 | 25.01 | 41067.8 | D |
| ILC6 | Maize | 25 | 27.90 | 1020.4 | D |
| Barley | 60 | 25.60 | 5024.7 | D | |
| Soybean | 10 | 29.03 | 1117.1 | D | |
| Flax | 5 | 26.73 | 6790.0 | D | |
| ILC7 | Durum+soft wheat | 20 | 27.42 | 8331.2 | D |
| Maize | 20 | 27.80 | 1132.7 | ND | |
| Barley | 20 | 26.86 | 1943.0 | D | |
| Soybean | 20 | 27.83 | 2389.3 | D | |
| Sunflower | 5 | 29.39 | 7715.6 | D | |
| Flax | 5 | 26.71 | 6728.0 | D | |
| Rice | 5 | 31.98 | 53.2 | D | |
| Cotton | 5 | 28.28 | 1599.7 | D | |