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. 2018 Jan 2;13(1):e0190465. doi: 10.1371/journal.pone.0190465

Fig 5. Immunostaining of β2 nAChR in WT mouse CV taste bud cells.

Fig 5

The images are merged images of DIC, DAPI, and secondary antibody fluorescence (Alexa Fluor® 488). (A) A low magnification image of mouse CV taste papilla section that shows preferential binding of the nAChR β2 antibody to the apical pole of CV taste bud cells (red arrows). (B) and (C) High resolution images of the CV taste bud cells showing preferential binding of the antibody to the apical pole of the TRCs. Some binding was also observed in the intracellular/basal compartment of TRCs. (D) No antibody binding was observed when the primary antibody step was omitted (NC). Horizontal bars = 10 μm.