Fig 3. Abscission occurs when DC3000 touches the AZ, however, long-distance signals are also sent from distal portions of the leaf to the AZ.

(A) Leaves infiltrated in indicated portions of the leaf shown 3 days after infection. The same samples are shown in the top and bottom panels where the top panels are imaged with reflected white light and the bottom panels are imaging YFP fluorescence. (B) Percent of cauline leaves to abscise three days after treatment. Data are mean ± s.e.m.; n = 7 biological replicates (one plant each); letters indicated different statistical quantities t-test P < 0.05. (C) Quantification of HAE-YFP fluorescence two days after infection while all leaves were still attached. Data are mean ± s.e.m.; n = 4 biological replicates (one plant each); letters indicate different statistical quantities t-test P < 0.05. Scale bar is 0.5 mm.