Fig 1. Survival of F. gigantica NEJs in RPMI-1640, DMEM and DME/F-12 culture media supplemented with 10% foetal bovine serum, 2% glucose and 25 mM HEPES.

NEJs showed higher % survival (≥ 80%) in RPMI-1640 which was lower in DMEM and DME/F-12 medium (≥75% and ≥72%), respectively at 3 weeks of culture. All flukes died by 28–30 days of culture.