Table 1. Sense and anti-sense primers tailored with T7 promoter sequence at 5' end (in bold) for in vitro transcription of dsRNA trigger against six target genes of F. gigantica and for irrelevant control dsRNA against Plasmodium falciparum KAHRP gene.
Primer Name | Sequence |
---|---|
FgSOD–F FgSOD-R |
5'-TAATACGACTCACTATAGGGATATCCGCGGGACCTCATTTCA AC-3' 5'- TAATACGACTCACTATAGGGCCAATAACTGAGTTCACTCCGG AG-3 |
FgCat B1+B3-F FgCat B1+B3-R |
5'- AATACGACTCACTATAGGGACCGACCGTATATGCATTCATT-3' 5'- TAATACGACTCACTATAGGGTTGGGGAACGGGTAGGGTAAAC-3' |
FgCat B-2-F FgCat B-2-R |
5'- TAATACGACTCACTATAGGGGGCGGCAGCCAGTGCAATGAGT-3' 5'- TAATACGACTCACTATAGGGT TGGTAAACATCCAGGGCTGAC-3' |
FgCat L1-D-F FgCat L-1 D-R |
5'- TAATACGACTCACTATAGGGTATAAGGCGAACAAGCCCGCCG-3' 5'- TAATACGACTCACTATAGGGGTTGCTCAGAGAATGAAGCACT-3' |
FgσGST-F FgσGST-R |
5'- TAATACGACTCACTATAGGGAATTCGCCTTCTGCTCACTTGT-3' 5'- TAATACGACTCACTATAGGGGTAATACTCCTCGTCCGTTTCACC-3' |
PfKAHRP-F PfKAHRP-R |
5'- TAATACGACTCACTATAGGGGCAAAAGAAGCAAGTACTTCTAA-3' 5'- TAATACGACTCACTATAGGGGCAGTTCCATCTTTAGATTGTAC-3' |