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. 2017 Dec 12;11(12):e0006109. doi: 10.1371/journal.pntd.0006109

Table 2. Primers designed for qRT-PCR estimation of the mRNA transcript knockdown of F. gigantica target genes post-RNAi.

Primer Name Sequence
FgSOD -F
FgSOD-R
5'-TGGAGATACAACGAATGGTTGT-3'
5'- CCAATAACTGAGTTCACTCCGG-3'
FgCat B1+B3-F
FgCat B1+B3-R
5'-TCCAGTTGTAGTTCGTGTTGG-3'
5'- TTGGGGAACGGGTAGGGTAAAC-3
FgCat B-2-F
FgCat B-2-R
5'- GTTGTGGTTCCTGTTGGGCCAC-3'
5'- AACCGGTTCGATTTTCCCAAGT-3'
FgCat L1-D-F
FgCat L-1 D-R
5'- GTTACTCTCACGCGGTATCCCG-3'
5'- AGCTCTTTCGTTCTTCCTAAAC-3'
FgσGST-F
FgσGST-R
5'- ATTCCGTGGGCGAGCAGAACC-3'
5'- GTAATACTCCTCGTCCGTTTCACC-3'
FgGAPDH-F
FgGAPDH-R
5'- GGCTGTGGGCAAAGTCATTC-3'
5'-AGATCCACGACGGAAACATCA-3'