Table 2. Primers designed for qRT-PCR estimation of the mRNA transcript knockdown of F. gigantica target genes post-RNAi.
Primer Name | Sequence |
---|---|
FgSOD -F FgSOD-R |
5'-TGGAGATACAACGAATGGTTGT-3' 5'- CCAATAACTGAGTTCACTCCGG-3' |
FgCat B1+B3-F FgCat B1+B3-R |
5'-TCCAGTTGTAGTTCGTGTTGG-3' 5'- TTGGGGAACGGGTAGGGTAAAC-3 |
FgCat B-2-F FgCat B-2-R |
5'- GTTGTGGTTCCTGTTGGGCCAC-3' 5'- AACCGGTTCGATTTTCCCAAGT-3' |
FgCat L1-D-F FgCat L-1 D-R |
5'- GTTACTCTCACGCGGTATCCCG-3' 5'- AGCTCTTTCGTTCTTCCTAAAC-3' |
FgσGST-F FgσGST-R |
5'- ATTCCGTGGGCGAGCAGAACC-3' 5'- GTAATACTCCTCGTCCGTTTCACC-3' |
FgGAPDH-F FgGAPDH-R |
5'- GGCTGTGGGCAAAGTCATTC-3' 5'-AGATCCACGACGGAAACATCA-3' |