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. 2017 Dec 18;13(12):e1006786. doi: 10.1371/journal.ppat.1006786

Fig 5. eCD4-IgG2 enhances ADCC activity of HIV-1 infected sera against reactivated latently infected cells.

Fig 5

OM-10.1 (A, C) or ACH-2 (B, D) cells lines were reactivated with TNFα (A, B) or vorinostat (SAHA) (C, D) for 24 hours prior to being mixed at a 10:1 effector to target ratio with an NK cell line expressing human CD16a in the presence of a 1:1000 dilution of serum 9121658 alone or in combination with 1 μg/mL of eCD4-IgG2, or CD4-IgG2, or VRC01-IgG2. ADCC responses, calculated based on intracellular p24 staining, were measured after a 5 hour incubation. Results are expressed as means +/- S.E.M. (n = 3). Data are representative of at least three independent experiments. Unpaired t-test, ***p<0.001, **p<0.01, *p<0.05, n.s. not significant.