Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) |
male C57BL/6J mice | The Jackson Laboratory | Stock#: 000664 RRID:IMSR_JAX:000664 |
|
| Genetic reagent (Mus musculus) |
male heterozygous Slc6a3Cre+/− (DAT-Cre) mice |
The Jackson Laboratory | Stock#: 006660 RRID:IMSR_JAX:006660 |
maintained on the C57BL/6J background |
| Genetic reagent (Mus musculus) |
male Ai9 reporter mice | The Jackson Laboratory | Stock#: 007909 RRID:IMSR_JAX:007909 |
maintained on the C57BL/6J background |
| Strain, strain background (Adeno-associated virus) |
AAV2.shRNA.U6.ShRLuc. CMV.eGFP.SV40 |
shRNA provided by Dr. Han-gang Yu (PMID: 19236845), packed into AAV2 at Penn Vector Core |
||
| Strain, strain background (Adeno-associated virus) |
AAV2.scramble.U6.ShRLuc. CMV.eGFP.SV40 |
Penn Vector Core | ||
| Strain, strain background (Adeno-associated virus) |
AAV2.CMV.PI.HCN2. WPRE.eGFP.SV40 |
HCN2 plasmid packed into AAV2 at Penn Vector Core |
HCN2 plasmid provided by Dr. Dane Chetkovich at Northwestern University |
|
| Strain, strain background (Adeno-associated virus) |
AAV2.CMV.PI.eGFP.WPRE.bGH | Penn Vector Core | ||
| Antibody | Mouse anti-TH | Santa Cruz Biotechnology | SC-136100, Lot: G1309, RRID:AB_2287193 |
monoclonal, 1:300 |
| Antibody | Rabbit anti-TH | Santa Cruz Biotechnology | SC-14007, Lot: C2707, RRID:AB_671397 |
polyclonal, 1:300 |
| Antibody | Rabbit anti-NeuN | Millipore | ABN78, Lot: 2702139, RRID:AB_10807945 |
polyclonal, 1:400 |
| Antibody | anti-mouse IgG Alexa Fluor 555 |
Cell Signaling | Stock #4409, RRID:AB_1904022 |
Goat anti-mouse, 1:300 |
| Antibody | anti-rabbit IgG Alexa Fluor 647 |
Invitrogen | A21245, Lot: 1445259, RRID:AB_141775 |
Goat anti-rabbit, 1:100 |
| Antibody | anti-mouse IgG Alexa Fluor 488 |
Cell Signaling | Stock #4408, RRID:AB_10694704 |
Goat anti-mouse, 1:300 |
| Antibody | anti-rabbit IgG Alexa Fluor 488 |
Cell Signaling | Stock #4412, RRID:AB_1904025 |
Goat anti-rabbit, 1:500 |
| Other | neurobiotin tracer | Vector Laboratories | SP-1120, RRID:AB_2336606 |
1.5% |
| Other | Texas Red avidin D | Vector Laboratories | A-2006, RRID:AB_2336751 |
1:100 |
| Software (Sigmaplot 11.2) | RRID:SCR_003210 |