Table 1. Anti-LigB mAb characterization summary.
Mean values for the dissociation constant (KD), FACS cell binding propensity (MFI), and lethal dose (LD50) are listed for mAbs against LigB1-7 (library C) and LigB7-12 (library V). Double and single domain specificities for mAbs are also noted.
| Anti-LigB mAbs | KD (μM) | Cell binding (MFI) | LD50 (μg/ml) | LigB domain specificity | |
|---|---|---|---|---|---|
|
Library C
(Anti-LigB1-7) |
Double domain | Single domain | |||
| C1 | 1.232 | 3497.5 | 15.29 | LigB1-2 LigB2-3 |
LigB2 |
| C5 | 0.896 | 4499.5 | 15.07 | LigB4-5 | LigB5 |
| C6 | 0.923 | 4644.5 | 13.76 | LigB4-5 | LigB5 |
| C7 | 0.848 | 2722.5 | 18.72 | LigB1-2 | LigB1 |
| C9 | 2.440 | 3530.0 | 19.56 | LigB1-2 | n.s. |
| C10 | 2.162 | 21.63 | LigB4-5 | n.s. | |
| C12 | 3.301 | 11.57 | LigB2-3 LigB4-5 |
LigB4 LigB6 |
|
| C13 | 1.166 | 4186.0 | 18.98 | LigB1-2 LigB4-5 |
n.s. |
| C23 | 2.916 | 3469.5 | 29.92 | LigB2-3 | n.s. |
| C22 | n.b. | 1121.5 | |||
|
Library V
(Anti-LigB7-12) |
Double Domain | Single Domain | |||
| V1 | 15.151 | 25.85 | LigB9-10 LigB10-11 |
LigB10 | |
| V2 | 1.391 | 2889.0 | 20.45 | LigB11-12 | LigB12 |
| V3 | 1.367 | 2568.5 | 16.19 | LigB7-8 LigB11-12 |
LigB7 |
| V10 | 0.738 | 3211.0 | 14.87 | LigB9-10 LigB10-11 |
LigB10 |
| V11 | 2.956 | 19.24 | LigB7-8 LigB11-12 |
LigB8 LigB12 |
|
| V12 | 9.574 | 2333.5 | 29.18 | LigB7-8 | LigB7 LigB9 |
| V13 | 7.064 | 2376.0 | 28.60 | LigB7-8 | LigB8 |
| V26 | 9.915 | 22.83 | LigB9-10 LigB10-11 |
n.s. | |
| V34 | 11.011 | 2665.0 | 25.28 | LigB7-8 | LigB7 LigB9 |
| V33 | n.b. | 910.5 | |||
| Source of relevant data |
Figure 3, Figure 3—figure supplement 1 |
Figure 4B, Figure 4—figure supplement 2 |
Figure 5, Figure 5—figure supplement 1 |
Figure 4A, Figure 4—figure supplement 1 |
|
n.b. no significant binding.
n.s. no significant binding partner was determined.