Table 1.

Clinical EAE scores

Score	Scoring Method	Observation
0	Hold mouse by the base of the tail	Unaffected; mouse can “helicopter” tail
0.5	Hold mouse by the base of the tail	Some loss of tail tone
1.0	Hold mouse by the base of the tail	Complete tail limpness, with no evidence of limb weakness
1.5	Hold mouse at base of the tail between index finger and thumb, resting the heel of your palm flat on a surface. Attempt to roll the mouse once by rolling its tail between your fingers.	Can roll mouse, but with some struggling
2.0	Hold mouse at base of the tail between index finger and thumb, resting the heel of your palm flat on a surface. Attempt to roll the mouse once by rolling its tail between your fingers.	No hind limb paralysis upon ambulation, but mouse fails to remain upright when the examiner attempts to roll the mouse
2.5	Hold mouse at base of tail and place its forepaws on edge of the cage. Be prepared to catch the mouse should it fall.	Climbs into cage with difficulty; normal ambulation
3.0	Hold mouse at base of tail and place forepaws on edge of cage. Be prepared to catch the mouse should it fall.	Inability to climb over cage edge; partial paralysis of hind limbs; waddles upon ambulation (but does not drag limbs)
3.5	Observe ambulation	Partial paralysis of hind limbs as evidenced by dragging one limb upon ambulation
4.0	Observe ambulation	Complete paralysis of both hind limbs; dragging body by forearms; still capable of moving around the cage
4.5	Observe mouse behavior	Responsive but not moving/stationary, listless, rapid breathing (consult institutional veterinarian; consider euthanasia)
5.0	Observe mouse behavior	Immobile and unresponsive; Moribund (immediate euthanasia recommended)