Skip to main content
. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Neurosci Methods. 2017 Apr 8;284:71–84. doi: 10.1016/j.jneumeth.2017.04.003

Table 2.

Troubleshooting

Step Problem Possible Reason Solution
Step 13 The emulsification mixing step does not produce a viscous solution Insufficient mixing Allow emulsification syringes to sit until the CFA and DPBS phases separate. Attempt to emulsify again. Repeat (1–3 times) until there is a noticeable and consistent shift in viscosity
Step 22 The emulsion is runny and loses its viscosity when loading 1mL syringes The emulsion is too warm Place the emulsion at −20°C for a minimum of 30 min. Keep the emulsion on an ice pack while loading.
Steps 28, 30 The emulsion pellets disperse following injection The emulsion is too warm Keep the 1 mL syringes on an ice pack or place at −20°C until the solution has become more viscous.
Disease onset/Clinic al scoring Symptoms do not occur or disease incidence is low Improper preparation or faulty/expired reagents Double-check all calculations, concentrations, sample preparations, and reagent expiration dates (especially PTx).
Improper MOG35–55 CFA pellet injection location Euthanize and dissect mouse to verify that the pellets are in proximity to the inguinal and axial lymph nodes. Adjust future injections accordingly.