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. 2018 Jan 2;12(1):014102. doi: 10.1063/1.5019728

FIG. 1.

FIG. 1.

Experimental overview. (a) The microfluidic device used in these experiments has a height of 7 μm. Here, a PC3 prostate cancer cell nucleus, labeled with Hoechst 33 342 fluorescent stain, is shown entering the constricted channel. Scale bar: 10 μm. (b) The length L(t) of the portion of the nucleus inside the constricted channel is measured in time. By dividing L(t) by the initial nuclear stress, the creep compliance function J(t) is obtained; see text for details. (c) A labeled PC3 nucleus is advancing into the constricted channel over time. Scale bar: 10 μm. (d) The creep compliance function J(t) for PC3 and DU145 prostate cancer cell line nuclei and a RWPE-1 prostate epithelial cell nucleus. J(t) follows a power law for approximately 2 decades in time, where each cell shown has a different exponent α and intercept A (PC3, α = 0.39, A = 2.2 × 10−3 Pa−1; DU145, α = 0.26, A = 2.5 × 10−4 Pa−1; RWPE-1, α = 0.33, A = 1.3 × 10−4 Pa−1).