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. 2018 Jan 2;12(1):014102. doi: 10.1063/1.5019728

FIG. 7.

FIG. 7.

Confirmation of chromatin decondensation by drug 5-AZA-2'-deoxycytidine (AZA). Fluorescence and inverted fluorescence images of nuclei stained with Hoechst 33 342 for control and treated cells: (a) PC3, (b) DU145, and (c) RWPE-1. Scale bar is 20 μm. (d) AZA reduces the average nuclear fluorescence intensity, and therefore, chromatin is less condensed. PC3 n = 331, PC3 AZA n = 217, p < 1 × 10−5. DU145 n = 296, DU145 AZA n = 291, p ≪ 1 × 10−6. RWPE-1 n = 303, RWPE-1 AZA n = 362, p ≪ 1 × 10−6. The two-sample Kolmogorov-Smirnov test was used for statistical comparisons. Data obtained from 3 independent samples and average intensities were taken from raw fluorescence images.