Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 2;148(12):123315. doi: 10.1063/1.5004606

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Author(s)

Published by AIP Publishing.

0021-9606/2018/148(12)/123315/9/$30.00

PMC Copyright notice

FIG. 2. — Qualitative assessment of smFRET dynamics using BVA. Proximity ratio (PR) histograms and BVA plots of σ(PR) versus PR show that (a) the free promoter is either characterized by a single PR population with no smFRET dynamics or with no PR population at all, due to quenching of FRET, and (b) RNAP bound to promoter DNA induces a unique PR subpopulation that exhibits smFRET dynamics. Depiction of differently labeled D-A constructs in free promoter (a) and RPo (b) states was generated by calculating D and A AVs (green and red surfaces, respectively) on top of DNA labeling positions in the context of RPo crystal structure (pdb code: 4XLN).