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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Kidney Int. 2017 Aug 23;93(1):128–146. doi: 10.1016/j.kint.2017.06.008

Figure 12. AQP2 protein is strongly expressed in cultured CCD cells in G0, but not when in the cell cycle.

Figure 12

dDAVP-treated (1 nM) mpkCCD cells were grown on a semi-permeable filter and synchronized in the different phases of the cell cycle (FCS withdrawal for G0 phase, PD0332991 for G1, Hydroxyurea for S and Nocodazole for G2/M phase), then immunoblotted for AQP2 and Cyclins D1, A, and B.