Table 10.
Statistical Methods
Data Type | Measure | Metrics | Purpose | Criterion |
---|---|---|---|---|
RNA-Seq | Fragments per kilobase exons for million mapped reads (FPKM) | Padj; z value | Descriptive; Strength of evidence | none |
Semi-Quantitative Immunoblotting of Kidney Tissue (Figure 1) | Normalized band density | P (unpaired t-test) | Testing null hypothesis | P<0.05 |
Semi-Quantitative Immunoblotting of Cultured Cells (Figures 7 and 12) | Normalized band density | P (ANOVA; Bonferroni) | Testing null hypothesis | P<0.05/n |
Enrichment of Genes from Cutated Lists in Vasopressin-escape Gene Lists | Number of transcripts | P (Chi-square) | Testing null hypothesis | P<0.05 |
Enrichment of Gene Ontology Terms in Vasopressin-escape Gene Lists | Number of transcripts | P (Fisher Exact; DAVID) | Testing null hypothesis | P<0.05 |
Cell Counts in Immunocytochemically Labeled, Microdissected Tubules | Number of cells | P (unpaired t-test) | Testing null hypothesis | P<0.05 |
Cell Counts of PCNA positive cells | Number of cells | P (Chi-square) | Testing null hypothesis | P<0.05 |
Cell Counts of high density DAPI-labeled nuclei | Number of cells | P (Fisher Exact) | Testing null hypothesis | P<0.05 |