Table 5.
Comparisons of cell populations in microdissected CCDs from rats undergoing vasopressin-escape compared with control rats.
| # Nuclei per mm length | % Pendrin-positive ICs | % Pendrin-negative ICs | % Total ICs | Ratio PCs:ICs | Ratio α-ICs: β-ICs | |
|---|---|---|---|---|---|---|
| Control 1 | 547 | 21 | 15 | 37 | 1.69 | 0.75 |
| Control 2 | 575 | 27 | 16 | 44 | 1.30 | 0.57 |
| Control 3 | 479 | 22 | 19 | 41 | 1.45 | 0.84 |
| Control 4 | 453 | 17 | 29 | 46 | 1.16 | 1.80 |
|
| ||||||
| Mean ± SE | 514 ± 29 | 22 ± 2 | 20 ± 3 | 42 ± 2 | 1.40 ± 0.11 | 0.99 ± 0.27 |
| Escape 1 | 449 | 20 | 21 | 41 | 1.40 | 1.16 |
| Escape 2 | 583 | 19 | 16 | 36 | 1.99 | 0.86 |
| Escape 3 | 555 | 21 | 16 | 39 | 1.55 | 0.77 |
| Escape 4 | 451 | 14 | 22 | 42 | 1.45 | 2.06 |
|
| ||||||
| Mean ± SE | 509 ± 35 | 19 ± 1 | 19 ± 2 | 40 ± 1 | 1.60 ± 0.13 | 1.21 ± 0.29 |
| p-Value | 0.93 | 0.26 | 0.82 | 0.33 | 0.30 | 0.60 |
A total of 35 microdissected CCDs were analyzed, 13 from Control rats and 22 from Escape rats. There were no statistically differences between two groups of animals (unpaired Student’s t-test, n=4). Percent of principal cells (PCs) may be estimated as 100 minus the percent of total ICs. IC, intercalated cell.