Table 9.
Antibody | Species | Approach | Dilution | Source | Reference | Expected size (non-glyc., glyc. kDa) |
---|---|---|---|---|---|---|
AQP2 | Rabbit | WB | 1:1000 | Knepper | 17 | 29, 35–50 |
AQP2 | Chicken | IC | 1:300 | Knepper | 2 | 29, 35–50 |
AQP3 | Rabbit | IC | 1:100 | Knepper | 11 | |
VDAC | Mouse | IC | 1:500 | Abcam ab14374 | ||
H-ATPase B/B2 subunit | Mouse | IC | 1:50 | Santa Cruz sc55544 | ||
PCNA | Rabbit | IC | 1:100 | Abcam ab18197 | ||
AQP2 | Rabbit | WB | 1:1000 | Deen | 5 | 29, 35–50 |
Cyclin D1 | Rabbit | WB | 1:1000 | Cell Signaling 92G2 | 36 | |
Cyclin A | Mouse | WB | 1:1000 | Thermo Fisher Scientific MA1-154 | 52 | |
Cyclin B | Mouse | WB | 1:1000 | Thermo Fisher Scientific MA5-14319 | 55 |
WB- western blotting, IC- immunohistochemistry; glyc. – glycosylated