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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Phytochemistry. 2017 Dec 22;146:36–46. doi: 10.1016/j.phytochem.2017.11.010

Figure 5.

Figure 5

Effect of parthenolide and estafiatin on activation-induced ERK1/2 phosphorylation in human primary T cells. Isolated human T cells were pretreated for 20 min with 1% DMSO or the indicated concentrations of parthenolide and estafiatin, followed by activation with human T-activator CD3/CD28 Dynabeads (bead-to-cell ratio 1:1) for 5 min, and the levels of ERK phosphorylation were evaluated using an ELISA for human phospho-ERK1/2. The data are presented as mean ± SD of triplicate samples. Statistically significant differences between cells treated with control DMSO versus DMSO + CD3/CD20 beads (ap<0.01) and between cells treated with DMSO + CD3/CD20 beads versus cells treated with the indicated lactones + CD3/CD20 beads (bp<0.01) are indicated. The results shown are representative of three independent experiments.