Fig. 4.

Immunofluorescence in RMM tumors (2 cm) for CD68 (a), CD163 (b) and merged (c): all CD163⁺ cells co-express CD68, arrowheads indicate CD68 single-positive cells. Immunofluorescence for MHC class II (d), CD68 (e) and merged (f): many MHC class II⁺ cells co-express CD68 (arrows). Immunofluorescence for MHC class II (g), CD163 (h) and merged (i): a few MHC class II⁺ cells co-express CD163 (arrows). Percentages of double positive cells in RMM tumors of 0.5, 1, 2 and 3 cm are indicated for CD68/CD163 (j); MHC class II/CD68 (k) and MHC class II/CD163 (l) double immunolabeling. Bars, 100 μm