Fig. 4.

Differential DUSP expression across the GB tumor microenvironment. The expression of DUSP family across discrete regions of the tumor microenvironment are shown. Data obtained from the Ivy GAP consortium are presented as normalized fragments per kilobase of transcript per million mapped reads (FPKM). Regions of GB biopsy samples including the “normal” leading edge (LE, n = 19), bulk cellular tumor (CT, n = 11), pseudopalisading cells around necrosis (CTpan, n = 40), and perinecrotic zone (CTpnz, n = 26) were identified using ISH panels prior to laser-capture microdissection. “Downregulated/No Change” and “Upregulated in Cellular Tumor” groups include DUSPs with unchanged/decreased or increased expressions in tumor structures (CT, CTpan, CTpnz) relative to “normal” LE tissues, respectively. The “Upregulated in Necrotic Core” group represents DUSPs with increased expression in only necrotic CTpan and CTpnz regions relative to LE. Statistical significance was determined using one-way ANOVA with Bonferroni correction to correct for multiple comparisons (ns = not significant, * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001)