Fig. 2.

Effect of SR11302 on the primary tumor of ex vivo 4D lung model seeded with H1299 cells. a Image of the primary tumor (left) and metastatic lesion (right) on day 25 of the 4D model seeded with H1299 without treatment showing tumor (brown) on both sides. d Image of the primary tumor (left) and metastatic lesion (right) on day 25 of the 4D model seeded with H1299 treated with SR11302. There is lack of visible tumor on the metastatic lesion side. b & e Hematoxylin and eosin image of the primary tumor. There is no difference in the cell morphology between the untreated (B) and treated (E) primary tumor. c & f Immunohistochemitry stain for Ki-67. There is no difference in proliferation index between untreated (C) and treated (F) 4D model seeded with H1299 (p = 0.33). g Relative gene expression of c-jun in primary tumor (PT) was significantly downregulated upon SR 11302 treatment (p < 0.001). h Total number of CTCs was less after after SR11302 treatment in 20 day period of cell culture on 4D model