Fig. 7.

Deacetylation of K126 on GKRP improves hepatic glucose uptake and glucose intolerance in diabetic obese mice. a–e Effect of adenovirus-mediated hepatic overexpression of GKRP-K126Q or GKRP-wild-type (GKRP-WT) on blood glucose (a) and plasma insulin (b) levels under free-feeding conditions and changes in blood glucose (c), plasma insulin (d), and hepatic 2-deoxyglucose (2-DG) uptake (e) levels during glucose administration (2 g/kg) in lean mice (n = 5). f–j Effect of adenovirus-mediated hepatic overexpression of GKRP-K126R or GKRP-WT on blood glucose (f) and plasma insulin (g) levels under free-feeding conditions and changes in blood glucose (h), plasma insulin (i), and hepatic 2-DG uptake (j) levels after glucose administration (1 g/kg) in HFD mice (n = 5). k–o Effect of adenovirus-mediated hepatic overexpression of GKRP-K126R or GKRP-WT on blood glucose (k) and plasma insulin (l) levels under free-feeding conditions and changes in blood glucose (m), plasma insulin (n), and hepatic 2-DG uptake (o) levels after glucose administration (1 g/kg) in db/db mice (n = 4). p Acetylation level of GKRP in db/db mice transfected with adenovirus encoding Flag-GKRP-WT or Flag-GKRP-K126R. Endo. endogenous GKRP, Exo. exogenous GKRP. *P < 0.05; Student’s t-test (a–o). Data in p are representative of at least three independent experiments. Error bars show s.e.m