Fig. 5.

Dnmt1 is the direct downstream target of microRNA let-7d-3p. a Relative expression of let-7d-3p in db/db HSCs (n = 6, *p < 0.05 vs. WT; ^# p < 0.05 vs. db/db). b Luciferase activity assay (n = 4, *p < 0.05 vs. control). Dnmt1 M1 represents mutant #1 and Dnmt1 M2 represents mutant #2. c Relative expression of let-7d-3p in WT HSCs following transfection with a let-7d-3p inhibitor (n = 6, *p < 0.05 vs. WT control). d Relative expression of Dnmt1 in WT HSCs following transfection of let-7d-3p inhibitor (n = 6, *p < 0.05 vs. WT control). e Relative expression of let-7d-3p in db/db HSCs following transfection with let-7d-3p mimic. f Relative expression of Dnmt1 in db/db HSCs following transfection of let-7d-3p mimic (n = 6, ^# p < 0.05 vs. db/db control). Results are expressed as means ± SEM. One way ANOVA was used for a. Two-tailed unpaired Student’s t test was used for b–f