Fig. 3.
The 95 a.a. N-terminal truncation enhances DNA translocation activity of RAD54. a The experimental scheme of the triple-helix displacement assay. The triple-helix forming oligonucleotide (TFO) is paired to the linearized pMJ5 plasmid. The asterisk indicates the 32P-label. b The kinetics of triple-helix (0.5 nM, molecules) displacement by RAD54 (5 nM) or RAD5496–747 (5 nM) was analyzed by electrophoresis in 1.2 % agarose gels. c Data from b are plotted as a graph. The values obtained in protein-free reaction (Supplementary Figure 1b) were subtracted from the values of RAD54-promoted reactions. Each experiment was repeated three times. Error bars represent the s.e.m.