Fig. 10.

Melatonin inhibited OxyHb-induced neuronal apoptosis. a Neuronal apoptosis in various groups were detected via PI and Annexin V double staining and flow cytometry analysis in vitro. b Bar graphs showing different conditions of neurons in various groups: PI−/Annexin V− represents survival neurons, PI+/Annexin V− represents necroptotic neurons, PI−/Annexin V+ represents apoptotic neurons, and PI+/Annexin V+ represents neurons with mixed damage. All data are displayed as mean ± SEM, with *P < 0.05 and ^& P < 0.05 considered significant; NS, no significant difference, n = 6