Fig. 6.

DNA damage indicator expression and immunofluorescence (IF) staining to visualize DNA-damaged cells around the hematoma in brain tissues 72 h post-ICH induction. a Western blot analysis examining XRCC1 and γ-H2AX levels in the sham, ICH, ICH + vehicle, and ICH + melatonin groups. b, c Relative XRCC1 and γ-H2AX levels were calculated based on densitometry analysis. The mean values of XRCC1 and γ-H2AX within the sham group were normalized to 1.0. All data are displayed as mean ± SEM, with *P < 0.05 and ^& P < 0.05 deemed as significant difference; NS, no significant difference, n = 6. d Representative IF staining to identify 53BP1-positive cells (green) and with nuclei fluorescently labeled with 4,6-diamino-2-phenyl indole (DAPI, blue). Arrows indicate 53BP1-positive cells; scale bar = 32 μm. e Percentage of 53BP1-positive cells around the hematoma in the brain. All data are displayed as mean ± SEM, with *P < 0.05 and ^& P < 0.05 deemed as significant difference; NS, no significant difference, n = 6