Table 3.
Parameters of variability and extraction recovery for 15d-PGJ2
| Intraday | Interday | ||||||
|---|---|---|---|---|---|---|---|
| Nominal concentration[pg mL−1] | Recovery [%] | Measured concentration [pg mL−1] | Accuracy [%] | Precision [% CV] | Measured concentration [pg mL−1] | Accuracy [%] | Precision [% CV] |
| 5 | 91.6 ± 4.6 | 5.3 ± 0.6 | 106.0 | 11.3 | 4.9 ± 0.3 | 98 | 6.1 |
| 150 | 88.6 ± 13.9 | 141.2 ± 11.5 | 93.9 | 8.1 | 136.7 ± 15.8 | 91.1 | 11.6 |
| 400 | 96.1 ± 6.1 | 461.9 ± 54.3 | 115.5 | 11.8 | 389.1 ± 57.2 | 97.3 | 14.7 |
Displayed is the intra- and interday accuracy/precision and the extraction efficiency based on the recovery. Accuracy is defined as the mean of the quantified concentration in percent of 3 spiked concentrations (nominal) in human artificial plasma samples. Precision is described as the CV of the mean concentration determined for 3 different concentrations (intraday, n = 4; interday, n = 4)