Table 2:

Primers used for mtDNA amplification

Primer sequence (5′-3′)	Primer position	Tm (°C)	Size (bp)	Gene
F: GCAGGGACCAACGAATGCT	4211–4230	59	330	tRNAIle, Gln, Met
R: CCTTCCTCACACTGGCACTTGTA	4540–4521
F: CCCTTACCACGCTACTCCTA	5461–5480	59	280	tRNAAla, Trp, Asp
R: GGCGGGAGAAGTAGATTGAA	5740–5721
F: CAAACACTTAGTTAACAGCT	5681–5700	59.5	300	tRNATyr, Cys, Asn
R: GCTCATGCGCCGAATAG	5980–5961
F: ATTAATTCCCCTAAAAATCT	8221–8240	57	250	tRNALys
R: TAGGTGGTAGTTTGTGTTTA	8470–8451